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Chenevert Architects

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´╗┐Nitric oxide synthase (NOS) inhibitors have been reported to increase as well as to decrease microvascular transport of macromolecules in a variety of models. This study was performed to determine whether the influence of NOS inhibition on albumin leakage was dependent on the fluorescent dyes used to label albumin. Ugg Boots Nordstrom Albumin leakage was assessed in rat Ugg Canada mesenteric venules during control conditions and after exposure to the NOS inhibitor NG-nitro-L-arginine methyl ester (L-NAME). Albumin was labeled with any one of four dyes: FITC, sulforhodamine 101 [Texas Red (TR)], dichlorotriazinyl aminofluorescein (DTAF), or Oregon Green 514 (OG). Superfusion with L-NAME (10(-4) M) was accompanied by an increase in leakage of FITC-labeled albumin (n = 12) but not of albumin labeled with DTAF (n = 10), TR (n = 10), or OG (n = 4). In vessels perfused with both FITC- and TR-labeled albumin (n = 12), superfusion with L-NAME increased leakage of FITC- but not TR-labeled albumin. In conclusion, albumin leakage responses to L-NAME differ among various fluorescent dyes. Therefore, caution is advised in comparison of albumin leakage results that utilize different fluorescent dyes.  

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Baton Rouge Office

315 Third Street, Suite A
Baton Rouge, Louisiana 70801
phone: (225) 334-9907
fax: (225) 334-9908

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New Orleans Office

8200 Hampson Street Suite 200
New Orleans, Louisiana 70118
phone: (504) 314-1404
fax: (504) 314-1406

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email: info@chenevertarchitects.com

 

 

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